Biohazard Research Guidelines for

Psychology Department Animal Research Laboratories

 

 

TOPICS COVERED IN THIS DOCUMENT:

 

·       Introduction: Responsibilities in Research and Teaching Labs

·       Biosafety Program Registration Requirements (UVA POLICY: XIV.D.1)

·       Biological Safety Cabinets

·       Fire Safety (UVA POLICY: XIV.K.1 & XIV.G.1)

·       Industrial Hygiene Program (UVA POLICY: XIV.L.1)

·       Laboratory Chemical Fume Hoods

·       Radioactive Material and/or Radiation Producing Equipment

 

Introduction: Responsibilities in Research and Teaching Labs

The Psychology Department has assigned Hazardous waste Management concerns in research labs to the faculty member responsible for them.  The faculty member will be responsible for ensuring that all occupants of the laboratory are properly trained by the Office of Environmental Health and Safety (OEHS) on the correct methods of Hazardous Waste Collection.  In the case of common use areas, the Department Chair will assign responsibility to one faculty member. In the case of teaching labs, the instructor of each lab will be responsible for the Hazardous Waste Program in that particular lab.  If Teaching Assistants are used on a day-to-day basis in the lab, the instructor should be aware that they too must be properly trained by OEHS in Hazardous Waste Handling and Collection Methods.  Teaching Assistants must be strongly urged to make sure all containers are labeled, capped when not in use, and properly stored. The instructor will be responsible for ensuring the clean up of the laboratory area and the end of the teaching session.

 

Biosafety Program Registration Requirements (UVA POLICY: XIV.D.1)

 

All Principal Investigators must register with the University of Virginia Institutional Biosafety Committee (IBC) by declaring the hazard potential of the agents manipulated in their respective laboratories.  In addition, those who conduct research with agents designated Biosafety Level 2, or above, must formally document the fact that their protocols and facilities do not jeopardize the health and well-being of themselves, their employees, or the general public.

 

Biosafety Level 2 agents and required follow-up activities are described in detail at the OEHS Biosafety Homepage at http://keats.admin.virginia.edu/bio.

 

 

Biological Safety Cabinets

 

Biological safety cabinets (which are frequently, though incorrectly, referred to as “Laminar Flow Hoods”) are certified according to prescribed criteria under a contract with a certified vender.  This contract is administered by the OEHS.  Because routine services are paid for out of research overhead funds, there are no additional fees assessed to the Principal Investigator.  Researchers are, however, expected to make there own arrangements for these services.  The toll-free number is available through the OEHS at 2-4909.

 

 

Chemical Safety and Waste Training (UVA POLICY: XIV.R.1)

 

Annual Chemical Safety and Waste Training is required for all faculty, staff and students who meet any of the following criteria:

1.      The person performs work in a laboratory.

2.      The person works with or around chemicals or chemical products (e.g. cleaning materials) in the performance of her/his job duties.

3.      The person generates HAZARDOUS WASTE (see definition below, under Hazardous Waste Collection and Disposal) at the University of Virginia.

OEHS provides Chemical Safety and Waste Training, free-of-charge, to any faculty, staff or student member identified above.

TRAINING RESOURCES

  1. Laboratories, Departments or other groups may schedule a "live" Chemical Safety and Waste Training session by calling OEHS at 982-4911.
  2. Persons may also complete their Chemical Safety Training online via the OEHS World Wide Web Site http://keats.admin.virginia.edu.

 

 

Fire Safety (UVA POLICY: XIV.K.1 & XIV.G.1)

 

  1. OEHS provide fire safety training for staff, students, and faculty call OEHS (982-4911) to schedule training.
  2. Read the evacuation plan for your building.  Ask questions if you have any.  Know what you are supposed to do in the event of a fire alarm or fire.
  3. Hallways are a means of egress, and therefore, are to be kept clear of all materials (UVA POLICY: XIV.K.1).  Special circumstances may temporarily override this, with and only with, the approval of our office.
  4. Access to electric panels, fire extinguishers, pull stations, and other safety equipment must not be blocked at any time.
  5. Extension cords are not to be used as permanent wiring.  Use power strips or get permanent wiring installed (UVA POLICY: XIV.G.1).
  6. Please read all equipment instructions and use the equipment for approved uses only.
  7. Keep egress ways in your labs clear
  8. Maintain at least an 18-inch clearance to sprinkler heads
  9. Keep combustible material accumulation to a minimum.  Recycle often.
  10. You must respond to fire safety inspection notices, hopefully after you have corrected problems related to your lab.
  11. If you have fire safety questions, call 982-4911 or email fire-safety@virginia.edu.

 

 

Hazardous Waste Collection and Disposal (UVA POLICY: XIV.Q.1)

 

OEHS manages all aspects of Hazardous Waste Collection and Disposal at UVA.  All Hazardous Waste must be reported to OEHS to ensure proper handling and disposal.  OEHS is available to assist UVA Departments/Laboratories with questions, concerns and compliance regarding Hazardous Waste Regulations.  If a Department/Laboratory is unsure about whether a waste is a Hazardous Waste, OEHS must be contacted to provide assistance with the classification of the waste.  All persons generating and/or collecting HAZARDOUS WASTE must receive annual training from OEHS (UVA POLICY XIV.R.1 – see above).

 

HAZARDOUS WASTE

Any waste or combination of wastes which pose a substantial, present or potential, hazard to human health or living organisms because such wastes are non degradable or persistent in nature or because they can be biologically magnified, or because they can be lethal, or because they may otherwise cause or tend to cause detrimental cumulative effects.

 

Hazardous (Chemical) Waste at the University of Virginia may include but is not limited to the following:

 

  1. Out of date chemicals.
  2. Waste from laboratory processes.
  3. Waste from maintenance processes.
  4. Waste from landscaping and turf management processes (e.g. fertilizers and pesticides).
  5. Waste from construction processes.
  6. Spent batteries, fluorescent light bulbs and ballasts.

 

HAZARDOUS WASTE COLLECTION GUIDELINES

The following guidelines MUST be followed during the collection of HAZARDOUS WASTE at the University of Virginia:

 

  1. All containers being used for HAZARDOUS WASTE collection must be labeled with the words "WASTE" or "HAZARDOUS WASTE".
  2. All containers used for HAZARDOUS WASTE collection must be kept closed AT ALL TIMES except when actually adding material to the container.
  3. No more than 55 gallons of HAZARDOUS WASTE may be accumulated in any given room/lab.

 

AVAILABLE RESOURCES (Call 2-4911 or http://keats.admin.virginia.edu)

To assist faculty, staff and students OEHS provides the following:

 

  1. Containers for HAZARDOUS WASTE collection.
  2. Labels for HAZARDOUS WASTE containers.
  3. Pick-up of HAZARDOUS WASTE containers.

 

 

Industrial Hygiene Program (UVA POLICY: XIV.L.1)

 

Consultation with OEHS is required if a Department/Laboratory has a work environment or performs work processes that can generate harmful air contaminants or involve other potentially hazardous activities (including nonionizing radiation, biohazards, high noise levels, confined space entry, temperature extremes, or excessive vibration).  OEHS will provide consultative services at no charge to the Department/Laboratory.  There may, however, be charges applied to the requesting Department/Laboratory if environmental samples are collected and subsequently analyzed by a certified commercial laboratory.  The fees will not exceed those charged by the commercial laboratory.

 

 

Laboratory Chemical Fume Hoods

 

OEHS provides annual inspection of this critical laboratory safety device.  The inspection includes an assessment of the hood’s capacity to efficiently capture and contain toxic compounds generated by research activities conducted within the device.  A sticker, which provides the survey results, along with their significance and recommendations for user precautions, is affixed to the face of the hood each year.  The sticker also describes steps to be taken in the event that the hood does not pass the performance test.

 

 

Radioactive Material and/or Radiation Producing Equipment

 

To use Radioactive Material and/or Radiation Producing Equipment at UVA, you must be an “Approved” User.  OEHS grants this approval.  Obtaining the approval is absolutely required.  The elements in the approval are as follows:

 

  1. Completing an application
  2. Training – either at a short course offered by OEHS, or by submitting proof of training from another institution.
  3. Successfully passing an examination.

 

No one at UVA is allowed to work with Radioactive Material and/or Radiation Producing Equipment without being an “Approved” User.

 

Annual Radiation & Chemical Safety Training is required for all Approved Users at UVA.

 

TRAINING RESOURCES

  1. Laboratories, Departments or other groups may schedule a "live" Radiation & Chemical Safety Training session by calling OEHS at 982-4911.
  2. Persons may also complete their Radiation & Chemical Safety Training online via the OEHS World Wide Web Site http://keats.admin.virginia.edu.

 

 

 

Regulated Medical Waste Management (UVA POLICY: XIV.I.1)

 

Any waste that meets the definition, see below, must be considered RMW. All sharps RMW must be disposed of using sharps containers. All non-sharps RMW must either be placed in clear autoclavable waste bags and steam sterilized in accordance with the Virginia RMW regulations (i.e. certain time, temperature and pressure, regular spore testing, recordkeeping, etc.), placed inside a properly constructed CMC, or in the case of the UVA Health Care Facilities, placed in a re-useable RMW container (i.e. large red rolling cart or Rubbermaid step-on can). All autoclaved RMW must be labeled as follows:

The autoclaved waste can then be disposed of as normal trash.

Once a CMC is full, the top must be taped closed and properly labeled with the generator’s name, building, room number, phone extension and date. In the Health Care Facilities, the CMC’s must be removed by UVA Environmental Services Department and transported to the appropriate storage site for pickup by the contracted RMW transporter. In all other areas, the CMC’s must be brought to the appropriate RMW storage area for your building (contact the Office of Environmental Health & Safety-OEHS for information on this location) where it will be removed and managed either by OEHS or by the Contracted RMW transporter.

Within the UVA Health Care Facilities, the re-useable RMW containers must be removed either by Hospital Environmental Services or by other specially assigned staff member. These containers must be labeled prior to removal with the location, contact name, phone number, and the date of closure. These containers must be brought to the applicable storage site (Contact Environmental Services Dept. for this location) for pick-up by the Contracted RMW transporter.

All pathological waste (non-RMW) must be packaged in 6-mil green polyethylene bags (available from Hospital Stores), twisted and taped closed, and then brought by the generator to the applicable cold storage area in your building (contact OEHS for this location). OEHS will remove this waste on a regular basis for treatment at the UVA incineration plant.

The disposal of RMW is both highly regulated and very costly. University of Virginia faculty, staff and students must use the utmost care to segregate all waste materials properly. No Radioactive or Hazardous Waste shall be placed within the RMW stream. Also, no normal unregulated trash or recyclable materials shall be put into RMW containers. Each UVA Department shall decide which individual materials qualify only as RMW and which must by treated differently. Training of individuals who make these determinations must be done as part of the annual OSHA Blood Borne Pathogens Regulations training, performed by the Hospital Epidemiology Department.

Regulated Medical Waste (RMW)
Any waste materials that are capable of producing an infectious disease by an organism likely to be pathogenic to healthy humans, such as the following:

  1. Discarded cultures, stocks, specimens, vaccines and associated items likely to contain pathogenic organisms.
  2. Discarded bio-safety level 2 agents and etiologic agents.
  3. Wastes from the production of biologicals and antibiotics likely to have been contaminated by pathogenic organisms.
  4. Free-flowing human blood, blood products, and body fluids, including items containing or visibly contaminated with blood.
  5. Human tissues or anatomical waste.
  6. Sharps likely to be contaminated with pathogenic organisms, and all discarded needles/syringes.
  7. Empty chemotherapy containers.
  8. Remains of animals intentionally infected with human pathogens, including carcasses, body parts, bedding and related wastes.
  9. Residue from the cleanup of RMW.
  10. Any solid waste that is contaminated by or mixed with RMW.

BIO-SAFETY LEVEL 2 AGENTS
Organisms present in the community that are associated with human diseases of varying severity.

ETIOLOGIC AGENTS
Organisms that cause a particular disease.

PATHOGENS
Disease causing agents.

SHARPS
Needles, scalpels, knives, glass, syringes, Pasteur pipettes and similar items having a point or sharp edge or that are likely to break during transportation and result in a point or sharp edge.

SHARPS CONTAINER
Plastic, highly needle puncture resistant waste container used for disposal of needles and associated syringes.

CONTAMINATED MATERIALS CONTAINER (CMC)
A customized corrugated cardboard box system with an integral red plastic bag and cardboard liner used within the UVA research departments and Health Sciences Center for disposal of non-sharp RMW.

PATHOLOGICAL WASTE
Animal carcasses that are not pathogenic to healthy humans (animals not infected with human pathogens), or human cadavers which have been embalmed such that they are no longer potentially pathogenic to healthy humans. These are not RMW.